Composite

Part:BBa_K3396002:Design

Designed by: Yuxin Liu   Group: iGEM20_NUDT_CHINA   (2020-10-26)


TRIM21-FRB


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 204
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1088
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 161
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

To fulfil out aqquire that controling the degradation, we come up with the idea that truncate the PRYSPRY domain of the TRIM21 and replace it with FRB, which bind with FKBP mediated by rapamycin.


Source

The original Trim21 is expressed in human. We did the truncation based on the protein domain information given in Uniprot and replaced it with FRB,which is from human mTOR(aa sequence).

References